Skip to main content
. Author manuscript; available in PMC: 2021 Jul 6.
Published in final edited form as: Dev Cell. 2020 Jun 8;54(1):117–131.e5. doi: 10.1016/j.devcel.2020.05.015

Figure 3. Scaling growth of the NMJ is independent of activity-dependent synaptic plasticity.

Figure 3.

(A-F) Confocal images of the NMJ on muscle 6/7 in w1118 (A), Mad (B), ether a go-go (eag), Shaker (Sh) (C), eag, Sh; Mad (D), w1118; C57-GAL4>UAS-InR-WT (E), and Mad; C57-GAL4>UAS-InR-WT (F) labeled with anti-HRP (red), anti-Syt (green), and anti-Dlg (blue). Scale bars, 30 μm. (G-L) Quantification of the muscle 6/7 MSA (G, J), number of boutons (H, K), and number of boutons normalized to MSA (I, L) of the NMJ on muscle 6/7. Mad is required for the increase in bouton number in eag, Sh double mutants, but not for the increase in bouton number in response to InR activation in muscle. n=23 (w1118), n=24 (Mad), n=13 (eag, Sh), n=20 (eag, Sh; Mad,), n=17 (w1118; C57>InR-WT), or n=19 (Mad; C57>InR-WT). ***p<0.0001; ns, not significant by unpaired Student’s t-test. See also Figure S2.