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. 2020 Jul 8;8:101. doi: 10.1186/s40478-020-00973-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — MYCN amplification detected by NGS and FISH studies. a.MYCN copy number from next generation sequencing studies. All cases were interrogated by the OCAv3 NGS panel to obtain comparable data except for case 7 which was interrogated by the custom PBTP as there was insufficient material to reanalyze the case using the OCAv3 platform. b. FISH analysis of the 8 MYCN amplified cases showing high signal amplification in all cases