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. 2020 May 27;9(6):460. doi: 10.3390/antiox9060460

Figure 2.

Figure 2

Neurites from wobbler motor neurons of the evolutionary phase (p20) are significantly shorter than wildtype cells after 14 DIV. (A) Exemplary images of dissociated motor neuronal cells of wildtype spinal cord of the evolutionary phase (p20) after 7, 10 and 14 DIV stained with anti-NFM antibody (green) and DAPI (nucleus, blue). (B) Exemplary images of dissociated motor neuronal cells of wobbler spinal cord of the evolutionary phase (p20) after 7, 10 and 14 DIV stained with anti-NFM antibody (green) and DAPI (nucleus, blue). (C) Neurite lengths of p20 motor neurons after 7, 10 and, 14 DIV. Constant uptrend of wildtype (WT) neurite lengths while wobbler (WR) neurites show a significantly loss in length over time in a sense of degeneration. Different development of the genotypes up to a significant difference in neurite length after 14 DIV can be observed. Data are provided as means ± SEM. Data were tested for significance using two-way ANOVA with a Sidak post hoc test. Significant differences are indicated by * p < 0.05, *** p < 0.001. N = 56–150. Scale bar = 50 µm.