Fig. 2.

T2*W MRI located the hemosiderin deposits of CMBs in vivo. a A large CMB region was visible in the old-aged mouse is labeled with * in the MRI slice shown. b The relationship found between CMBs presence visible in T2*W MRI and the age of mice in months was significant in the linear regression model (p < 0.001; R² value of 0.8716). The volume of CMBs was measured in vivo manually as the area of CMBs on an image multiplied by the slice thickness. Resolution (140 µm³ isotropic) was minimized to prevent partial volume effects. Quantification indicated increasing presence of CMBs in relation with aging (# indicates a p value is less than 0.01 by ANOVA followed by Tukey multiple pairwise-comparisons). The white bar represents a scale bar with size of 5 mm