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. 2020 Jul 8;13:340. doi: 10.1186/s13071-020-04191-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Maximum likelihood tree (− ln = 2009.56) based on partial sequences of the 70 kDa heat-shock protein gene, including sequences obtained in this study from naturally (red circle and bolded) and experimentally (green square and bolded) infected hosts. Tamura’s 3-parameter model was applied, using a discrete Gamma distribution and invariant sites. The robustness of the phylogeny was tested with 1000 bootstrap pseudoreplicates and numbers at the nodes represent the bootstrap values > 50%. The scale-bar indicates the number of substitutions per site. Sequences obtained in this study are identified by isolate number (e.g. 43201)