Table 2.
Cryptosporidium species and genotypes from this study, detected by amplification of small subunit ribosomal rRNA (SSU), actin, 70 kDa heat-shock protein (HSP70) and 60 kDa glycoprotein (gp60) gene fragments in juvenile common ostriches (Struthio camelus) on commercial farms in the Czech Republic
| Farm No. | No. of positive/no. of screened | ID of positive animal | Microscopical positivity (opg) | Genotyping at the gene loci | |||
|---|---|---|---|---|---|---|---|
| SSU | Actin | HSP70 | gp60 | ||||
| 1 | 5/40 | 43201 | No | C. ubiquitum | C. ubiquitum | – | XIIa |
| 43205 | No | C. ubiquitum | C. ubiquitum | – | XIIa | ||
| 43210 | No | C. ubiquitum | C. ubiquitum | – | XIIa | ||
| 43223 | No | C. ubiquitum | C. ubiquitum | – | XIIa | ||
| 43228 | No | C. ubiquitum | C. ubiquitum | – | XIIa | ||
| 2 | 0/64 | – | – | – | – | – | – |
| 3 | 3/50 | 44782 | Yes (8000) | C. ornitophilus n. sp. | C. ornitophilus n. sp. | C. ornitophilus n. sp. | – |
| 44790 | No | C. ornitophilus n. sp. | C. ornitophilus n. sp. | C. ornitophilus n. sp. | – | ||
| 44796 | Yes (12,000) | C. ornitophilus n. sp. | C. ornitophilus n. sp. | C. ornitophilus n. sp. | – | ||
| 4 | 4/50 | 43545 | Yes (12,000) | C. ornitophilus n. sp. | C. ornitophilus n. sp. | C. ornitophilus n. sp. | – |
| 43551 | No | C. ornitophilus n. sp. | C. ornitophilus n. sp. | C. ornitophilus n. sp. | – | ||
| 43587 | Yes (6000) | C. ornitophilus n. sp. | C. ornitophilus n. sp. | C. ornitophilus n. sp. | – | ||
| 43588 | Yes (18,000)a | C. ornitophilus n. sp. | C. ornitophilus n. sp. | C. ornitophilus n. sp. | – | ||
aAnimal serving as a source of oocysts for transmission studies
Note: Infection intensity of Cryptosporidium spp. is expressed as the number of oocysts per gram of faeces (opg)