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. 2020 Jul 8;8:102. doi: 10.1186/s40478-020-00983-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Astrocytes can take up RBC-EVs preferentially at endfeet in vivo. a-d Representative images of brain slices of striatum following peripheral injection of DiI-EVs and labeling with GFAP (marker for astrocytes) and Kir4.1 (marker for astrocytic endfeet). Note that DiI-EV co-localized with GFAP (a-b) and Kir4.1 (c-d) (Scale bar, 10 μm). e Analysis of the percentage of DiI-EVs colocalizing with MAP 2, Kir4.1 or IBA1 labeled cells in brain slices of LPS pre-administered WT mice or A53T mice (n = 5 for LPS pre-administered WT mice; n = 4 for A53T mice). f-h Graphs show analysis of the percentage of DiI-EVs colocalizing with MAP 2 (marker for neurons), Kir4.1 (marker for astrocytic endfeet) or IBA1 (marker for microglia) labeled cells in cortex (CTX, (f)), striatum (STR, (g)) and midbrain (MIDB, (h)). Note that increased percentage of DiI-EVs were colocalized with Kir4.1 at striatum in A53T mice compared with the LPS pre-administered WT mice (g; means + S.E.M; n ≥ 4; **p < 0.01 by One-way ANOVA test)