Fig. 6.

circCELSR1 regulated ovarian cancer cell activities via BRD4. a SKOV3 and OVCAR4 cells were transfected with pcDNA3.1-BRD4 and mRNA level was evaluated by qRT-PCR. b SKOV3 and A2780 were transfected with pcDNA3.1-BRD4 and protein level of BRD4 were evaluated by Western blotting. c SKOV3 and A2780 were transfected with shcircCELSR1 or/and pcDNA3.1-BRD4, and colony formation assay were performed, and (d) quantification of colony formation was shown. e SKOV3 and A2780 were transfected with shcircCELSR1 or/and pcDNA3.1-BRD4, and cell apoptosis were evaluated by FACS, and (f) the quantification of apoptosis rate was shown. g SKOV3 and A2780 were transfected with shcircCELSR1 or/and pcDNA3.1-BRD4, and cell migration were measured by wound healing assay, and (h) quantification of cell migration was shown. i SKOV3 and A2780 were transfected with shcircCELSR1 or/and pcDNA3.1-BRD4, and cell invasion was measured by invasion assay, and (j) invasion rate was shown. All the results were shown as mean ± SD (n = 3). * p < 0.05 and ** p < 0.01