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. 2020 Jul 8;20:632. doi: 10.1186/s12885-020-07073-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Negative expression of CCR6 and CCR8 in OSCC. a Representative images of CCR6 and CCR8 staining in OSCC tissues and adjacent healthy tissues (magnification 100×). b and c Results of western blot showed that CCR6 and CCR8 were rarely expressed in OSCC tissues, adjacent healthy tissues, OSCC cells, and HOK cells. The full-length blots are presented in Supplementary Fig. S2. d and e Immunofluorescence staining of CCR6 and CCR8 (red) and nuclei (blue) in oral cancer cells (HSC-6, CAL27 and SCC9) and HOK cells (magnification 200×)