Fig. 5.
The CCR5 ablated SupT1-R5 cells gain selective advantage over unmodified cells. a The SupT1-R5 cells were transduced with lenti-CRISPR/AsCpf1-#4/#5 or control lentivirus at MOI = 30 for 4 days, the treated cells were then infected with R5-tropic HIV-1YU-2 at MOI = 0.5. The HIV-1 titer in supernatant of each cells at day 1, 7, 14 post-infection were determined by p24 gag ELISA kit. b The cleavage of CCR5 fragments at day 0, 7, 14 post-infection were analyzed by T7E1. The data shown were the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01;***P < 0.001; Statistical analysis determined using unpaired t-test