Fig. 4.

Aortic endothelium-dependent vasodilation is impaired in 2-kidney, 1-clip (2K1C) mice fed high-salt diet. A and B: mean ± SD % relaxation in response to acetylcholine (A) or sodium nitroprusside (SNP) (B) of aortae from sham-operated or 2K1C mice fed 0.1% or 4.0% NaCl (n = 6 or 7 per group). C and D: box plot diagrams of EC₅₀ (C) and E_max (D) of acetylcholine vasodilatory responses. A 2-way ANOVA with Bonferroni post hoc test for multiple comparisons revealed significant differences between groups including a higher EC₅₀ but lower E_max of 2K1C mice fed 4.0% NaCl. *P < 0.05.