Effect of Saturating Photosynthetic Active Radiation on Guard Cell Starch Metabolism and Stomatal Kinetics.
(A) Normalized whole-plant recordings of gs–ginitial in dark-adapted (30 min) plants in response to 2-h L, 2-h D, 2-h L, and 2-h D (L, light; D, darkness). Plants were illuminated with 400 μmol m−2 s−1 white light. The gs values were normalized to values at the EoN. Number of measured plants per genotype n ≥ 3; values presented are means. WT, wild type.
(B) Normalized whole-plant recordings of A-Ainitial values from plants under the same light regime as given in (A). Values for A were normalized to values at the EoN. Number of measured plants per genotype n ≥ 3; values presented are means.
(C) Guard cell starch dynamics of plants under the same light regime as given in (A). Each value represents mean ± se of three biological replicates of >110 individual guard cells obtained from three independent experiments. Different letters indicate statistically significant differences among time points for the given genotype for P < 0.05 determined by one-way ANOVA with post hoc Tukey’s test.