Figure 3.

GR24^ent-5DS Induces Interaction between KAI2 and SMXL2 in Vivo.

(A) Association of HA-KAI2 with GFP-SMAX1, GFP-SMXL2, GFP-SMXL5, and GFP-SMXL6 revealed by Co-IP assay (IP) in wild-type (Col-0) protoplasts in the absence or presence of 100 µM GR24^ent-5DS.

(B) Interaction between HA-KAI2 and GFP-SMXL2 revealed by Co-IP assay (IP) in wild-type (Col-0) protoplasts in the absence or presence of 100 µM KAR₁, GR24^ent-5DS, GR24^5DS, or GR24^4DO.

The HA-KAI2 recombinant protein was detected with anti-HA monoclonal antibody; the GFP-SMAX1, GFP-SMXL2, GFP-SMXL5, and GFP-SMXL6 fusion proteins and GFP were detected with anti-GFP monoclonal antibody. All experiments were repeated at least three times with similar results.