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. 2020 May 12;32(7):2271–2291. doi: 10.1105/tpc.19.00022

Figure 3.

Figure 3.

The Differential Effects of ARR1 and ARR12 on Shoot Regeneration Are Determined by Distinct Protein Functions and Expression Patterns.

(A) to (F) Shoot regeneration from root explants incubated for 14 d on SIM, including root explants of arr1 arr12 (A), arr12 (B), ARR12pro:ARR1 arr12 (ARR1 expressed under the control of the ARR1 and ARR12 promoters; [C]), Col-0 (D), arr1 (E), and ARR1pro:ARR12 arr1 (ARR12 expressed under the control of ARR1 and ARR12 promoters) plants (F).

(G) to (J) Shoot regeneration from root explants incubated for 14 d on SIM, including root explants from Col-0 (G), 35Spro:ARR12 transgenic (lines no. 1 and no. 2; [H] and [I]), and 35Spro:ARR1 transgenic plants (J).

(K) and (L) Number of regenerated shoots after 14 d of culture. Data represent mean ± sd (n = 24). ***Differ at P < 0.001 (Student’s t-test). Scale bars = 0.5 cm. Two independent transgenic lines were analyzed for each genetic background, which exhibited similar phenotypes ([C], [F], [H], and [I]).