Figure 7.

TRIPP Is Required for the Membrane Association of a TRAPPII-Specific Subunit.

(A) pTRS120:TRS120-GFP transgenic plants showing that TRS120 localizes to intracellular compartments in the wild type (WT), but not in tripp-1. n = 5 seedlings. Bar = 10 µm.

(B) Immunoblot analysis of pTRS120:TRS120-GFP transgenic plants in the wild-type (WT) and tripp-1 background using anti-GFP antibody. Ponceau S staining was used as the loading control.

(C) Root cells of the wild-type (WT) and tripp-1 plants expressing pTRS120:TRS120-GFP. TRS120-GFP localizes to the cell plate in the WT, but not in tripp-1. n = 6 seedlings. TRS120-GFP localized to the cell plate in all WT cells (n = 20 cytokinetic cells) but in only 12% of tripp-1 cells (n = 8 cytokinetic cells). Bar = 5 µm.

(D) TRS120-GFP localizes to the leading edges (white arrows) of expanding cell plates in the wild type (WT), whereas a diffuse cytosolic cloud is detected in the tripp-1 background. n = 6 seedlings. Bar = 5 µm.

(E) Incomplete cross walls are observed in tripp-1 root cells (6.5% ± 0.5% incidence in root tips). n = 9 seedlings. Bar = 10 µm.

(F) Representative three-dimensional rendering of incomplete cross walls in two adjacent cells of the tripp-1 mutant. n = 9 seedlings. Inset shows two cells (1 and 2) selected for 3D rendering. Bar = 10 µm.