. 2020 May 5;32(7):2424–2443. doi: 10.1105/tpc.20.00044

Table 1. The 14 Arabidopsis TRAPP components identified by SILIP-MS.

Gene ID	TRAPP Subunits Identified by TRS33 Immunoprecipitation from Arabidopsis Roots							Yeast Homolog	Human Homolog
	Forward			Reciprocal			Protein Name
	Peptide No.	% Cov	L/H Ratio	Peptide No.	% Cov	L/H Ratio	Protein Name
AT1G51160	5	41.4	4.47	4	28.4	0.41	BET5	BET5	TRAPPC1
AT1G80500	3	28.9	4.13	3	28.9	0.29	TRS20	TRS20	TRAPPC2
AT2G20930	6	52.9	3.79	5	52.9	0.39	TCA17	TCA17	TRAPPC2L
AT5G54750	12	55.4	11.4	12	54.3	0.18	BET3^a^,^b	BET3	TRAPPC3
AT5G02280	5	42.6	2.72	—	—	—	TRS23	TRS23	TRAPPC4
AT5G58030	5	22.6	2.48	2	10.3	0.37	TRS31^a^,^b	TRS31	TRAPPC5
AT3G05000	11	61.3	7.7	11	60.7	0.07	TRS33^a^,^b	TRS33	TRAPPC6
AT5G16280	16	11.7	7.27	13	9.5	0.15	TRS85^a	TRS85	TRAPPC8
AT5G11040	32	32.7	3.62	23	25.6	0.40	TRS120/VAN4^a^,^b	TRS120	TRAPPC9
AT5G54440	33	31	4.19	13	12.3	0.44	TRS130/CLUB^b	TRS130	TRAPP10
AT5G65950	20	17.6	7.76	12	11.2	0.18	TRAPPC11	—-	TRAPPC11
AT4G39820	15	35.8	8.06	8	21.3	0.24	TRAPPC12	—	TRAPPC12
AT2G47960	14	37.1	2.84	14	33.3	0.52	TRS65	TRS65	TRAPPC13
AT3G17900	22	30.4	3.54	19	24.8	0.39	TRIPP	—	—
	TRAPP Subunits Identified by TRS33 Immunoprecipitation from Arabidopsis Shoots
AT1G51160	4	34.9	14.7	2	14.2	0.16	BET5	BET5	TRAPPC1
AT1G80500	1	8.9	12.9	2	20	0.10	TRS20	TRS20	TRAPPC2
AT2G20930	6	63.6	3.9	4	36.4	0.36	TCA17	TCA17	TRAPPC2L
AT5G54750	12	55.4	30.2	11	46.8	0.07	BET3^a^,^b	BET3	TRAPPC3
AT5G02280	6	51.8	7.1	—	—	—	TRS23	TRS23	TRAPPC4
AT5G58030	4	29.2	11.9	6	29.7	0.14	TRS31^a^,^b	TRS31	TRAPPC5
AT3G05000	12	74.0	37.9	8	52.6	0.04	TRS33^a^,^b	TRS33	TRAPPC6
AT5G16280	16	12.7	10.3	13	8.6	0.12	TRS85^a	TRS85	TRAPPC8
AT5G11040	24	22.5	5.5	19	18.6	0.23	TRS120/VAN4^a^,^b	TRS120	TRAPPC9
AT5G54440	23	20.2	4.7	18	17.1	0.27	TRS130/CLUB^b	TRS130	TRAPP10
AT5G65950	27	22.8	12.8	13	12.4	0.12	TRAPPC11	—	TRAPPC11
AT4G39820	9	24.3	15.3	10	24.3	0.11	TRAPPC12	—	TRAPPC12
AT2G47960	14	32.4	5.2	12	30.5	0.26	TRS65	TRS65	TRAPPC13
AT3G17900	19	28.6	3.8	17	21.1	0.32	TRIPP	—	—

SILIP-MS was performed using TRS33:TRS33-4MycHis/trs33-1 complemented plants (roots and shoots were analyzed separately). The number of unique peptides (Peptide No.), percentage of sequence coverage (% Cov), and ratio of signal intensities between samples labeled with light (L, ¹⁴N) and heavy (H, ¹⁵N) isotopes (L/H ratio) are shown for two biological replicates, in which the Col-0 control (forward) or the TRS33-MycHis sample (reciprocal) was labeled with ¹⁵N. Dashes indicate no data.

Subunits previously identified in a proteomic study using isolated TGN compartments (Drakakaki et al., 2012).

Subunits previously identified by label-free IP-MS of AtTRS130 (Rybak et al., 2014). Names of yeast and human homologs are based on published work (Riedel et al., 2018; Thomas et al., 2018).