Figure 6.

The Impact of the RNAi Transgene on the Transcriptome.

Read counts of B. napus genes were ranked according to their expression levels in WTS line #581 (A) or transgenic line #580 (B); only the 30 highest ranking genes are shown. The genes associated with the highest read counts in the WTS line were mostly identified as encoding storage protein (napin or cruciferin). These represented ∼50% of the counts in WTS line #581 but only 4% in transgenic line #580. In the transgenic line, 50% of the read counts are contained in 614 of the highest ranking genes. For gene identifiers and further information, see Supplemental Data Set 2J. n = 3 biological repeats, each comprising pooled RNA extracted from 10 embryos. The significance threshold was set as 5% for the adjusted P value and a more than fourfold change in transcript abundance. Asterisks indicate that means differed significantly between the WTS and transgenic lines.