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. 2020 Apr 23;32(7):2307–2324. doi: 10.1105/tpc.19.00689

Figure 4.

Figure 4.

Altered Lignin Deposition in the Roots of WRKY15OE and WRKY15-EAR Transgenic Seedlings.

(A) Lignin accumulation in 7-d-old Col-0, WRKY15OE (line no. 8), and WRKY15-EAR (line no. 38) roots was detected by phloroglucinol-HCl staining. Images were taken using a Nikon microscope.

(B) Basic fuchsin (BS) staining of lignin in the roots of 7-d-old Col-0, WRKY15OE (line no. 8), and WRKY15-EAR (line no. 38) seedlings. DIC and fluorescence images were photographed using a Nikon C2 Plus LSCM. The white long bracket represents a gap in protoxylem vessels. Scale bar = 50 μm.