Table 1. High Confidence ASTREL Orthogroups Absent in all 5 Species (S. polyrhiza, Z. marina, U. gibba, G. aurea, and A. officinalis).

Arabidopsis Gene Names Within Orthogroup	Identification Method	Pfam Domains	Known Role in Immunity	Other Roles
Lipase-like: EDS1.1, EDS1.2, PAD4, SAG101	Galaxy group 16353	Lipase_3	Downstream signaling of NLRs (Lapin et al., 2019; Wagner et al., 2013; Cui et al., 2017; Zhu et al., 2011)	Crosstalk in drought pathway (Chini et al., 2004)
	Dicot_5571	EDS1_EP
	Monocot_8566
RNLs: NRG1, NRG1.2, NRG1.3, ADR1, ADR1-L1, ADR1-L2, ADR1-L3, RPW8	Galaxy group 4516	RPW8, NB-ARC, LRR	Downstream signaling of NLRs. (Bonardi et al., 2011; Castel et al., 2018; Qi et al., 2018; Lapin et al., 2019; Wu et al., 2019)	Initial stomatal closure for drought tolerance (Chini et al., 2004)
	Dicot_3070
	Monocot_8024
SDR4	Dicot_3663B	adh_short_C2	No	N/A
SDR5	Monocot_4965
ASPG2	Dicot_8371	TAXi_N​, TAXi_NC	No	N/A
	Monocot_7573
EDL3	Dicot_10185	F-box_like	No	Regulator of ABA signaling, osmotic stress-responsive (Koops et al., 2011).
	Monocot_9077

N/A, not applicable.