Figure 1.

PNP-A Is Highly Upregulated in lsd1.

(A) Transcript levels of PNP-A in 17- and 19-d-old wild-type (WT) and lsd1 plants grown under CL were obtained from our previous RNA-sequencing analysis (Lv et al., 2019).

(B) Transcript levels of PNP-A shown in (A) were confirmed by RT-qPCR.

(C) Wild-type (WT) and npr1 plants grown under CL were sprayed with 0.5 mM SA solution (+SA) or with distilled water (–SA), and leaf samples were harvested 12 h after the treatment. The expression levels of PNP-A were examined using RT-qPCR.

(D) Expression levels of PNP-A in wild-type (WT), lsd1, and lsd1 npr1 (l/n1) plants grown under CL were analyzed by RT-qPCR at the indicated time points. For the RT-qPCR analyses in (B), (C), and (D), ACT2 was used as an internal standard. The data represent the means of three independent biological replicates. Error bars indicate sd. Lowercase letters indicate statistically significant differences between mean values (P < 0.01, one-way ANOVA with post hoc Tukey’s honestly significant difference [HSD] test; Supplemental Data Set).