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. 2020 Jul 9;21:41. doi: 10.1186/s12865-020-00370-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Inhibition of EGR2 suppresses Th1 differentiation of naïve CD4⁺ T cells from both MRL and MRL-lpr mice. Naïve CD4⁺ T cells from MRL and MRL-lpr mice at 6–8-week-old were transfected with NC (red) and EGR2 (blue) dsiRNA, respectively. Twenty-four hours after transfection, the cells were cultured at Th1 condition for 3 days. The Th1 cell culture supernatant were collected for ELISA; the cells were stimulated with PMA and ionomycin for 5 h for intracellular flow analysis. (a & g) The representative histogram plots show the EGR2 and IFNγ expression in the Th1 cells differentiated from NC and EGR2 DsiRNA treated MRL (a) and MRL-lpr (g) naïve CD4⁺ T cells. (b-e and h-k) The summary graphs show the percentage of EGR2 expressing cells (b, h), EGR2 expression intensity (c & i), the percentage of IFNγ expressing cells (d, j), IFNγ expression intensity (e, k) in Th1 cells differentiated from DsiRNA treated MRL and MRL-lpr naïve CD4⁺ T cells. (f & l) ELISA analysis of IFNγ in Th1 culture supernatant of DsiRNA-treated MRL (f) and MRL-lpr (l) naïve CD4⁺ T cell. Graphs show means ± SEM (n = 4 each). Paired student t-tests (NC vs EGR2 DsiRNA); *, p < 0.05, **, p < 0.01, and ***, p < 0.001