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. 2020 Jul 8;20:294. doi: 10.1186/s12935-020-01334-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — TMPO facilitates the biological functions of TC cells. a The interaction among miR-498, TMPO-AS1 and TMPO was testified by RIP assay. b Luciferase reporter assay was used to confirm that miR-498 bound with TMPO-AS1 or TMPO. c RNA pull-down assay was utilized to validate that miR-498 could bind with TMPO-AS1 or TMPO. d The mRNA expression of TMPO was detected by QRT-PCR in transfected cells. e The efficiency of sh-TMPO#1/2 was appraised by QRT-PCR. f–k Functional assays evaluated the influence of TMPO. **p < 0.01. n.s.: no significance