Figure 1. Gel filtration chromatography of Naja oxiana venom on Sephadex G-50. An amount of 180 mg of crude venom was separated on a 2 x 90 cm column previously equilibrated with phosphate buffer pH 7.5. Five milliliter samples were collected at a flow rate of 50 mL/h and their absorbance was measured at 280 nm. Four well-separated fractions of interest (F1, F2, F3 and F4) were taken forward for analysis.
