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. 2020 Jun 19;26:e20190099. doi: 10.1590/1678-9199-JVATITD-2019-0099

Figure 3. Polyclonal phage ELISA of phage selected in successive rounds of panning against fraction F3. Results from selections from library I (HI) and J (HJ) are shown. The rounds of selection are as designated on the top of the image. Overnight coating of F3 (30 µg/mL). Around 1013 phages into the first well of each column, then 10-fold dilution down the plate. Adding anti-M13/HRP to detect binding of virus to the target. TMB was added as substrate for HRP. Reactions were stopped with sulfuric acid (yellow).

Figure 3.