Fig. 4.

Enhanced cytokinin signaling in abci20 abci21.

(a) Representative root tip images of multiple TCS:GFP-expressing wild type and abci20–1/21 lines grown in the absence or presence of 20 nM tZ. Scale bar = 20 μm.

(b) TCS:GFP fluorescence intensity measured in the root tips of the wild type or abci20–1/21 transgenic lines expressing TCS:GFP. Seeds were germinated and grown on ½ MS-agar media supplemented with or without 20 nM trans-zeatin. Images were taken at 8 days after seed sowing. The fold increase of TCS:GFP level in tZ-treated root tips over in non-treated control root tips was calculated. The combined results (mean ± SD) of two independent experiments (total 7 biological replicates) are given. Different alphabets indicate that the means are significantly different between genotypes by Tukey’s Honest Significant Difference test at p ≤ 0.01.