TABLE 3.

The amplification program using the primer pairs described in Table 2.

Primer	Amplification program	References
adc5F/adc5R	94°C 5 min; 2 cycles of 94°C 45 s, 62°C 1 min, 72°C 1 min; followed by 20 cycles, when annealing temperature was decreased by 0.5°C in every other cycle: 94°C 45 s, annealing from 61 to 52°C 1 min, 72°C 1 min; 10 cycles of 94°C 45 s, 52°C 1 min, 72°C 1 min with a final extension at 72°C for 10 min.	Wunderlichová et al. (2013)
agm4F/agm4R	94°C 5 min; 2 cycles of 94°C 45 s, 62°C 1 min, 72°C 1 min; followed by 20 cycles, when annealing temperature was decreased by 0.5°C in every other cycle: 94°C 45 s, annealing from 61 to 52°C 1 min, 72°C 1 min; 10 cycles of 94°C 45 s, 52°C 1 min, 72°C 1 min with a final extension at 72°C for 10 min.	Wunderlichová et al. (2013)
adiA3F/adiA3R	94°C 5 min; 2 cycles of 94°C 45 s, 62°C 1 min, 72°C 1 min; followed by 30 cycles, when annealing temperature was decreased by 0.5°C in every other cycle: 94°C 45 s, annealing from 61 to 47°C 1 min, 72°C 1 min; 3 cycles of 94°C 45 s, 47°C 1 min, 72°C 1 min with a final extension at 72°C for 10 min.	Wunderlichová et al. (2013)
PUT1-F/PUT1-R	95°C 5 min, 25 cycles of 95°C 45 s, 55°C 1 min, 72°C 2 min with a final extension at 72°C for 10 min.	de las Rivas et al. (2006)
CAD1-F/CAD-R	95°C 5 min, 25 cycles of 95°C 45 s, 55°C 1 min, 72°C 2 min with a final extension at 72°C for 10 min.	de las Rivas et al. (2006)
106/107	95°C 5 min, 25 cycles of 95°C 45 s, 54°C 1 min, 72°C 1 min with a final extension at 72°C for 10 min.	de las Rivas et al. (2005)