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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Exp Eye Res. 2020 May 18;196:108064. doi: 10.1016/j.exer.2020.108064

Figure 6. Differences in αSMA and, Fibronectin-EDA, and Fibronectin (FN) in Segmental TM Outflow Regions by Immunofluorescence.

Figure 6.

Immunofluorescence was performed for αSMA, FN-EDA, and FN. FN-EDA antibodies were unsuitable for Western blot. Using aqueous angiography-determined angle sections, elevated αSMA and FN-EDA were demonstrated in LF sections. However, FN was not statistically significantly different comparing LF and HF sections. Relative fluorescence was quantitatively evaluated using six sections each from at least three different donors and compared with an unpaired two-tailed Student’s t-test. Scale bar = 100 microns. HF = high-flow and LF = low-flow. αSMA = alpha-smooth muscle actin, FN-EDA = EDA isoform of fibronectin, and FN = fibronectin.