TABLE 1:

Biochemical reactions and parameter values for the polarity module.

Biochemical reactions
Reaction	Rate constant	Value	Reference
	k1a	10 s−1	(Goryachev and Pokhilko, 2008; Savage et al., 2012)
	k1b	10 s−1	(Goryachev and Pokhilko, 2008; Savage et al., 2012)
	k2a	0.16 μM−1 s−1	(Savage et al., 2012)
	k2b	0.63 s−1	(Savage et al., 2012)
	k3	0.35 μM−1 s−1	(Savage et al., 2012)
	k4a	10 μM−1 s−1	(Goryachev and Pokhilko, 2008; Savage et al., 2012)
	k4b	10 s−1	(Savage et al., 2012)
	k5a	144 s−1	(Dyer et al., 2013)
	k5b	20.8 s−1	(Dyer et al., 2013)
	k7	10 μM−1 s−1	(Dyer et al., 2013)

Model specifications
Parameter	Value	Comments	Reference
Cell radius	2.5 μm		(Savage et al., 2012)
Membrane to cytoplasm volume ratio (η)	0.01		(Savage et al., 2012)
Membrane diffusion constant	0.0045 μm2 s−1		(Dyer et al., 2013)
Cytoplasmic diffusion constant	Assumed well-mixed		(Savage et al., 2012)
Internal membrane diffusion constant	Assumed well-mixed		(Savage et al., 2012)
Mean [Cdc42] in cell	1 μM	39415 molecules in 65.4 μm3	(Goryachev and Pokhilko, 2008; Savage et al., 2012)
Mean [BemGEF] in cell	0.017 μM	670 molecules in 65.4 μm3	(Goryachev and Pokhilko, 2008; Savage et al., 2012)