FIGURE 1:

Characterization of phosphosite-specific antibodies against P-domain phosphorylation sites in CCTα and  CCTβ. (A) Alignment of human and rat CCTα and CCTβ2 sequences showing the location of phosphorylated serine (red) and tyrosine (green) residues, and the phosphopeptides (yellow boxes) used to generate antibodies against pS315+pS319 and pY359+pS362 phosphosites in human CCTα and CCTβ. Corresponding sequences for rat CCT are shown (green boxes). (B, C) Peptides with the amino acid sequences shown were synthesized on cellulose membranes using the SPOT-synthesis technique and each macroarray was probed with anti–CCTα/β-pS319 (B) or anti–CCTα-pY359+pS362 (C) as described in Materials and Methods.