TABLE 2:

Summary of Axin’s ability to recruit E3 ligase components.

Proteins examined	No colocalization	Partial ­colocalization	Complete ­colocalization	Total number of cells analyzed	Number of ­experiments
Axin + Slimb	5	94	41	140	17
Axin + SkpA	48	4	0	52	6
Axin + Cul1	36	6	0	42	6
AxinΔRGS + Slimb	23	4	0	27	6
AxinΔβcat + Slimb	0	6	13	19	4
SrtAftRGS + Slimb	7	3	0	10	3
Axin + NTerm-Slimb	15	34	1	50	7
Axin + CTerm-Slimb	18	26	7	51	7

Summary of Axin’s ability to recruit E3 Ligase components. Genes encoding the wild-type or mutant proteins indicated were transfected into SW480 cells. No colocalization, no recruitment of protein into Axin puncta. Partial localization, protein recruited into a subset of puncta with substantial levels remaining in the cytoplasm. Complete colocalization, all Axin puncta have recruited its partner protein into the puncta, and cytoplasmic levels are reduced. Total number of cells analyzed represents cells imaged. The number of experiments equals the number of independent times cells were transfected with the labeled protein combinations.