(A) Summary of the different types of interactions between Axin and Dsh, including number of cells imaged. Strong overlap in localization, puncta are simple in shape and Axin and Dsh substantially overlap, resulting in yellow puncta. Transition to Separation, Axin and Dsh still exhibit substantial overlap but there are also regions of distinct localization. Segregation, Axin and Dsh proteins no longer appear to overlap although they often dock next to one another. Number of experiments equals the number of independent times cells were transfected with the labeled protein combinations. (B) Degree of colocalization observed at different Axin to Dsh transfection ratios. Ratios reflect Axin and Dsh plasmid concentrations used for transfections. The higher the Axin:Dsh ratio, the more complete the colocalization.
