Pros	Cons
Characterization and quantification of exosomes isolated from small amount of plasma	If the exosomal preparation from body fluids are not well purified, co-isolated contaminating biomolecules could result in a high level of background (biological noise), thus altering the results
Easy to perform	Nonspecific binding
High selectivity of specific exosomal antigen	Availability of suitable antibodies
Non expensive technique	The use of specific capture antibodies determines the quantification and characterization of a single sub-preparation of total exosomes preparation that carry the target protein(s), with following underestimation of the results (e.g., only CD9 +, only CD81 +, etc.)
Non-invasive technique
Simultaneous large-scale analysis of samples (up to 96 samples)
Exosomes capture facilitates the washing steps necessary to eliminate all components/debris not specifically linked to the capture antibody
Quantification of disease markers
Suitable for diagnostics