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Representative MRM ion chromatograms of stable isotope labelled and light MNs standards. A-C) show MRM chromatograms for PITC-derivatized MN-d3, NMN-d3, and 3-MT-d4, at (A) matrix blank, (B) LLOQ (40 pg/mL), and (C) ULOQ QC (2 ng/mL). (D) MRM chromatograms for a PITC-derivatized DBS sample fortified to 2 ng/mL with MN, NMN, and 3-MT.
