Table 1.
Optimized MRM transitions for PITC derivatized MNs on a QTrap 6500+. EP = Entrance Potential DP = Declustering potential; CE= Collision energy; CXP= Cell exit potential.
| Analyte | Precursor (m/z) | Product (m/z) | EP | DP | CE | CXP |
|---|---|---|---|---|---|---|
| NMN(-H2O) | 301.1 | 134.1 | 10 | 120 | 35 | 9 |
| NMN(-H2O) | 301.1 | 166.1 | 10 | 50 | 25 | 9 |
| NMN-d3(-H2O) | 304.1 | 137.1 | 10 | 120 | 35 | 9 |
| NMN-d3(-H2O) | 304.1 | 169.1 | 10 | 50 | 25 | 9 |
| MN(-H2O) | 315.1 | 180.1 | 10 | 120 | 28 | 9 |
| MN | 333.1 | 180.1 | 10 | 50 | 25 | 9 |
| MN-d3(-H2O) | 318.1 | 183.1 | 10 | 120 | 28 | 9 |
| MN-d3 | 336.1 | 183.1 | 10 | 50 | 25 | 9 |
| 3-MT | 303.1 | 151.1 | 10 | 120 | 25 | 14 |
| 3-MT | 303.1 | 119.0 | 10 | 120 | 25 | 14 |
| 3-MT-d4 | 307.1 | 155.1 | 10 | 120 | 25 | 14 |
| 3-MT-d4 | 307.1 | 123.0 | 10 | 120 | 25 | 14 |