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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: FASEB J. 2019 Nov 26;34(1):754–775. doi: 10.1096/fj.201901316RR

FIGURE 6.

FIGURE 6

INSR regulates mitochondrial polarization in hTCEpi cells. hTCEpi cells and HBECs were treated with siRNA oligonucleotides targeting INSR in KBM for 24 hours. A,B, Mitochondria were stained with MTG (green) and TMRE (red) to measure morphology and polarization, respectively. A, After INSR knockdown in hTCEpi cells cultured in KBM, mitochondrial morphology took on a ring/donut shape (mitochondrial fragmentation). Some hyperpolarized mitochondria were also detectable. 2X zoomed image of hTCEpi cells after INSR knockdown also shown. B, In HBECs, there were no changes in mitochondrial morphology and/or polarization with or without INSR. Scale bar: 10 μm. n = 3