Fig. 1. MiR-1165-3p inhibited Th2 differentiation. CD4⁺ T cells were cultured in Th2-cell conditions for 5 days and were transfected with miR-1165-3p enhancer or blank lentivirus on day 1. (A) MiR-1165-3p was significantly increased in Th2 cells infected with lentivirus-encoding miR-1165-3p (enhancer), (B, C) analysis of type 1 cytokines (IFN-γ and IL-12) and type 2 cytokines (IL-4, IL-5 and IL-13). (D-F) The Th1 and Th2 master transcription factors T-bet and GATA-3 were measured by qRT-PCR, western blotting and intracellular flow cytometry. Data are representative of independent experiments with similar results (n = 3–6).

MiR-1165-3p, microRNA-1165-3p; Th, T helper type; IFN, interferon; IL, interleukin; qRT-PCR, quantitative reverse transcription polymerase chain reaction; MFI, mean fluorescence intensity.

^*P < 0.05; ^†P < 0.01; ^‡P < 0.001.