Fig. 1.

Comparison of human and mouse NuRD complexes. A) and B). Proteins associated with MBD3/NuRD in hiPSCs (A) or mEpiSCs (B) were identified by immunoprecipitation and mass spectrometry. Proteins significantly associating with MBD3 are indicated, with NuRD component proteins indicated in red. The human data comprise three independent immunoprecipitations from one preparation of nuclear extract, while the mouse data comprise three independent immunoprecipitations each from nuclear extract preparations made from two independent cell lines. C) Overlap of proteins significantly associating with MBD3 in hiPSCs, Mbd3 in mEpiSCs, and Mta1, 2 or 3 in mouse ESCs (taken from (Burgold et al., 2019)) is displayed. The numbers in parentheses indicate the number of significantly enriched proteins in each experiment. D) Relative enrichment of indicated proteins normalised to the bait protein for each experiment: for mouse, ESC data (a combination of mass spectrometry experiments using Mta1, Mta2 and Mta3 as bait, taken from (Burgold et al., 2019); blue) were normalised to two MTA proteins, while for both the EpiSC (purple) and hiPSC (red) experiments the data were normalised to one MBD3 protein. Error bars represent standard deviation of three (hiPSCs), six (mEpiSCs) or nine (mESCs) replicates. Asterisks indicate situations where the protein enrichment was not significant in this cell type, but the stoichiometry is displayed for comparison. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)