| Gene | Forward primer | Reverse primer | Reference |
|---|---|---|---|
| CRB3 | AGGTCAAAGACGCCCG | TGAAGGCAAAACAGTGCTATTC | |
| FOXA2 | GGGAGCGGTGAAGATGGA | TCATGTTGGCTCACGGCGGCGTA | |
| FOXG1 | TCACGAAGCACTTGTTGAGG | AGGAGGGCGAGAAGAAGAAC | |
| INADL | GTGATGCCCTTGGAATCAGT | CTGCTCCTCTGTGTCTTCTG | |
| SOX2 | GGACAGTTACGCGCACAT | GCTGGTCATGGAGTTGTACT | |
| SOX17 | GGCGCAGCAGAATCCAGA | CCACGACTTGCCCAGCAT | |
| ZEB1 | CTGACTGTGAAGGTGTACCA | GTACATCCTGCTTCATCTGC | Jiang et al. (2018) |
| Ascl1 | CCTCTTAGCCCAGAGGAACA | GTCACTCTTCTCGTGTCTGG | |
| Bcam | GGTGATAGCAAAGGTCCAGG | CCGTTTCGGTACCATGTGAT | |
| Ccn2 | ATCTCCACCCGAGTTACCAA | TTTCATGATCTCGCCATCGG | |
| Cdh1 | GGCTTCAGTTCCGAGGTCTA | TCTCCAGCTTGTGGAGCTTT | |
| Cdkn2a | GGTTCTTGGTCACTGTGAGG | GTTCGAATCTGCACCGTAGT | |
| Dusp4 | GAGGAAAGGGAGGATTTCCA | GTACCTCCCAGCACCAATGA | |
| Dusp9 | AGAACGAAGCGGAGGCTA | AATCAGAGCTCAAGCACAGG | |
| Epcam | CCGGGCAGACTCTGATTTAC | CGGCTAGGCATTAAGCTCTC | |
| Lefty2 | ACACGCTGGACCTCAAGGAC | GCAGGTCCAGGTACATCTCC | |
| Sox3 | TTGCTGTTTAGCTTTGCTCG | TCAACTGCAACAGAAGAACC | |
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