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. 2020 Jul;46:101868. doi: 10.1016/j.scr.2020.101868

Fig. S1.

Fig. S1

Figure S1, Related to Figure 2: p63 expression at 6 weeks of differentiation after IWP2 and BMP4 treatment for different durations (0–4 day and 4–7 day). (A) FACS based on p63 expression at 6 weeks of differentiation. The plots are representative of 4 independent samples at 6 weeks of differentiation. Representative phase contrast images are at 6 weeks of differentiation (scale bar, 100 μm). Cells were treated with IWP2, BMP4, or IWP2 + BMP4 (I+B) from 0 to 4 days or from 4 to 7 days. Observation of SEAM zone 3 at 6 weeks of differentiation indicated by plus (+); absence indicated by a minus (−). (B) Quantitative gene expression of p63 for surface ectoderm marker. Data shown as the mean ± SD (n = six independent experiments). (C) The number of p63-EGFP+ cells by FACS analysis, (D) the total cell number, (E) The number of p63+/PAX6+ cells by cytospin analysis. (F) Differentiation efficiency of p63+/PAX6+ cells. Black line with asterisk indicates a statistical difference between two treatment groups (*p < 0.05, and **p < 0.01). A dashed line indicates a normalized value based on DMSO controls.