FIGURE 2.

Transfection efficiency of Schwann cells (SCs) with miR-124-containing vector. (A) miR-124 expression in SCs and ASs was significantly lower than that in the normal spinal cord according to qRT-PCR analysis (n = 3, ****p < 0.0001). (B) SCs showed green fluorescence 48 h after transfection with lentivirus. No significant cell morphology change was noted. The third figure is the magnification of the white dotted box in the middle one (first and second, scale bar = 100 μm; third, scale bar = 20 μm). (C) miR-124 expression in SCs after transfection for 48 h, 96 h, and 7 days were increased fourfold than those in NC and WT (n = 4, ****p < 0.0001).