Table 1. Variant calling results for vaginal microbiome samples processed at different truncation lengths with a consistent input of 346526 quality filtered reads.

Truncation length	Number of OTU	Number of NN	% OTU single hit to cpnDB_nr	% NN alignments in the correct direction
50 bp	189	150	71.4	83.6
100 bp	186	121	83.9	95.7
150 bp	189	107	90.5	100
200 bp	197	105	92.9	100
250 bp	206	105	91.3	100
300 bp	208	99	93.8	100