(A-F) Cardiac function in adult female ovariectomized (OVX) (n = 3) and sham-operated (n = 4) C57Bl/6NCrl mice was assessed by trans-thoracic echocardiography one week prior to treatment (Pre-ISO), and again after 2 weeks of daily subcutaneous injections of 10 mg/kg isoproterenol (Post-ISO). (A) Representative images from parasternal short axis view of the heart acquired in M-Mode. Effects of ISO on (B) ejection fraction, (C) fractional shortening, (D) LV volume in end-systole, (E) LV volume in end-diastole, (F) cardiac output, and (G) LV mass. *p<0.05, compared to before treatment of the same surgical status by matched two-way ANOVA with Sidak’s post-hoc analysis.