Fig 2. Peroxide-induced perturbations to peptide metabolism.

a, Heatmap showing the average fold change for all identified peptides at each time point after treatment of trophozoite-infected cultures (P. falciparum 3D7 strain) with OZ277, OZ439 and DHA. Values represent the average of at least three biological replicates, expressed relative to the average untreated control value (at least seven biological replicates) for that respective time point. Putative haemoglobin peptides are shown in red text. b, Representative time profiles showing the progressive depletion in abundance of selected putative haemoglobin-derived peptides after peroxide treatment of trophozoite-stage parasites (3D7). Values are the average fold change (± SD) relative to the untreated control of at least three biological replicates. c, Time-dependent decrease in the abundance of the four haemoglobin-derived peptides consistently depleted following peroxide treatment in ring infected cultures (3D7). Values are the average fold change (± SD) relative to the untreated control of four biological replicates. d, Heatmap of the peptides that were altered in abundance (≥ 1.5-fold relative to the untreated control) following peroxide treatment in the K13-wildtype artemisinin sensitive (Cam3.II^rev) and K13-mutant artemisinin resistant (Cam3.II^R539T) parasite lines. Data shown are the average for three or five biological replicates expressed relative to the average for the untreated control from the same parasite line. Putative haemoglobin peptides are shown in red text.