Table 2. Oligonucleotides used to generate the Mlh3^-/-, Pms1^-/- and Pms2^-/- mESCs.

Name	Oligo sequence (5' to 3')
Mlh3_gRNA-F	CACCGTTCTTCAACACACTGGCCCA
Mlh3_gRNA-R	AAACTGGGCCAGTGTGTTGAAGAA
Mlh3_ssODN	TCTATCAGATGACGTAAAAACCAAGTTGCGTTCCGGTTTAGCCATAAGCTTCTAGGGCTAGCGTGATGAAGAACTTACCCTTAACAGTATTGATGCTGAAGCAACATGTGTGGCCA
Pms1_gRNA-F	CACCGCAGAGTTCCAGATCACAGGA
Pms1_gRNA-R	AAACTCCTGTGATCTGGAACTCTG
Pms1_ssODN	TTCCATGTTGCCCATGACAGCAGTTCCCAGAACCGACATTAGAGGGATCCTAAGATCAGGAACACTGCTTTACTGCCAAATAACTGCCTAGGGAGAAGAAAACATAGATGTGTTGTAGCACA
Pms2-gRNA1-gibson	GAAAGGACGAAACACCGAGAGTTTGCCGACCTCACGCGTTTTAGAGCTAGAAATAGC
Pms2-gRNA2-gibson	TTTCTAGCTCTAAAACCGTGGTTTATGCTTTACTACGGTGTTTCGTCCTTT
Pms2-ssODN	CTTTCTACTCTCTTTCAGCTCTGAAACATCACACATCTAAGATTCAAGAGTTTGCCGTGCTTTACTACTGAAGTATCTTGACTCCGAGCTTTAATTCTCT
scaffold-U6prom	GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTCCGATCATGGGTCGAACGTTACGCAGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCG

Note: the underlined bases indicate the gRNA sequences and the bases in bold indicate the mutations introduced in the ssODNs to generate stop codons in all 3 reading frames.