Table 1. Summary of Canonical Gene-set Enrichment Analysis (GSEA) of differentially expressed transcripts between male G. m. morsitans tsetse fly antennae and head transcriptomes.
| Functional Database | Tissue | Annotation | Statistics | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Name | Class | Tissue | Process ID | Description | General Function | Size | L | ES | NES | P Value | FDR |
| Gene | Biological | Head | GO:0050953 | Sensory perception of light stimulus | Vision | 59 | 22 | 0.898 | 1.883 | 0.000 | 0.000 |
| Ontology | Process | GO:0007186 | G-protein coupled receptor signaling pathway | Vision | 162 | 56 | 0.796 | 1.869 | 0.000 | 0.000 | |
| GO:0032101 | Regulation of response to external stimulus | Vision | 101 | 8 | 0.801 | 1.833 | 0.000 | 0.000 | |||
| GO:0010927 | Cellular component assembly involved in morphogenesis | Muscle activity | 108 | 18 | 0.773 | 1.765 | 0.000 | 0.001 | |||
| GO:0042440 | Pigment metabolic process | Vision | 115 | 18 | 0.735 | 1.692 | 0.000 | 0.004 | |||
| GO:0009628 | Response to abiotic stimulus | Vision | 360 | 36 | 0.682 | 1.689 | 0.000 | 0.003 | |||
| GO:0003012 | Muscle system process | Muscle activity | 27 | 12 | 0.879 | 1.676 | 0.000 | 0.005 | |||
| GO:0044057 | Regulation of system process | Neuropeptide muscle regulations | 48 | 12 | 0.795 | 1.661 | 0.000 | 0.007 | |||
| GO:0043473 | Pigmentation | Vision | 103 | 18 | 0.706 | 1.610 | 0.003 | 0.025 | |||
| GO:0006730 | One-carbon metabolic process | Vision | 15 | 5 | 0.910 | 1.607 | 0.000 | 0.024 | |||
| GO:0003013 | Circulatory system process | Neuropeptide regulations | 40 | 12 | 0.784 | 1.590 | 0.005 | 0.032 | |||
| Antennae | GO:0044782 | Cilium organization | Mechanoreception | 62 | 22 | -0.839 | 2.170 | 0.000 | 0.000 | ||
| GO:0031503 | Protein complex localization | Mechanoreception | 30 | 12 | -0.849 | 1.903 | 0.000 | 0.001 | |||
| GO:0007606 | Sensory perception of chemical stimulus | Chemo-sensation | 124 | 57 | -0.628 | 1.806 | 0.000 | 0.006 | |||
| GO:0035218 | Leg disc development | Growth/differentiation | 87 | 15 | -0.665 | 1.781 | 0.000 | 0.007 | |||
| GO:0030705 | Cytoskeleton-dependent intracellular transport | Mechanoreception | 66 | 11 | -0.676 | 1.751 | 0.000 | 0.012 | |||
| GO:0030031 | Cell projection assembly | Mechanoreception | 112 | 35 | -0.624 | 1.742 | 0.005 | 0.011 | |||
| GO:0031099 | Regeneration | Repair/response to stress | 18 | 4 | -0.828 | 1.711 | 0.000 | 0.015 | |||
| Cellular | Head | GO:0019898 | Extrinsic component of membrane | Vision | 72 | 11 | 0.870 | 1.891 | 0.000 | 0.000 | |
| Component | GO:0016028 | rhabdomere | Vision | 34 | 17 | 0.955 | 1.886 | 0.000 | 0.000 | ||
| GO:0043292 | Contractile fiber | Muscle activity | 50 | 20 | 0.871 | 1.822 | 0.000 | 0.000 | |||
| GO:0015629 | Actin cytoskeleton | Vision/Muscle activity | 99 | 18 | 0.794 | 1.807 | 0.000 | 0.000 | |||
| GO:0098796 | Membrane protein complex | Vision | 233 | 10 | 0.690 | 1.689 | 0.000 | 0.001 | |||
| GO:0098858 | Actin-based cell projection | Vision | 22 | 4 | 0.861 | 1.600 | 0.002 | 0.012 | |||
| GO:0031984 | Organelle sub-compartment | Vision | 86 | 9 | 0.684 | 1.515 | 0.007 | 0.046 | |||
| Antennae | GO:0005929 | Cilium | Chemo-sensation/ Mechanoreception | 80 | 30 | -0.846 | 2.256 | 0.000 | 0.000 | ||
| GO:0031252 | Cell leading edge | Chemo-sensation | 52 | 28 | -0.811 | 2.005 | 0.000 | 0.000 | |||
| GO:0005815 | Microtubule organizing center | Mechanoreception/Muscle activity | 111 | 20 | -0.666 | 1.849 | 0.000 | 0.001 | |||
| Molecular | Head | GO:0005516 | Calmodulin binding | Vision/Muscle activity | 43 | 6 | 0.834 | 1.706 | 0.000 | 0.009 | |
| Function | Antennae | GO:0005549 | Odorant binding | Chemo-sensation | 49 | 35 | -0.843 | 2.170 | 0.000 | 0.000 | |
| Pathway | KEGG | Head | dme04745 | Phototransduction—fly—Drosophila melanogaster (fruit fly) | Vision | 25 | 14 | 0.954 | 1.772 | 0.000 | 0.000 |
| Analysis | Panther | Head | P00057 | Wnt signaling pathway | Vision | 62 | 8 | 0.827 | 1.749 | 0.000 | 0.000 |
| P00031 | Inflammation mediated by chemokine and cytokine signaling pathway | Vision/Muscle activity | 26 | 5 | 0.895 | 1.716 | 0.000 | 0.002 | |||
| P00044 | Nicotinic acetylcholine receptor signaling pathway | Vision/Muscle activity | 38 | 9 | 0.845 | 1.705 | 0.000 | 0.002 | |||
| P00016 | Cytoskeletal regulation by Rho GTPase | Vision/Muscle activity | 21 | 4 | 0.911 | 1.680 | 0.000 | 0.004 | |||
| P00004 | Alzheimer disease-presenilin pathway | Vision/Muscle activity | 25 | 5 | 0.848 | 1.593 | 0.005 | 0.026 | |||
| P00012 | Cadherin signaling pathway | Muscle activity | 25 | 3 | 0.839 | 1.585 | 0.003 | 0.025 | |||
| P00042 | Muscarinic acetylcholine receptor 1 and 3 signaling pathway | Vision/Neuropeptide regulations | 20 | 7 | 0.836 | 1.568 | 0.011 | 0.033 | |||
| P04374 | 5HT2 type receptor mediated signaling pathway | Vision | 18 | 7 | 0.841 | 1.567 | 0.014 | 0.030 | |||
| P00028 | Heterotrimeric G-protein signaling pathway-rod outer segment phototransduction | Vision | 5 | 3 | 0.991 | 1.563 | 0.000 | 0.031 | |||
| Reactome | Head | R-DME-1852241 | Organelle biogenesis and maintenance | Vision | 38 | 4 | 0.904 | 1.778 | 0.000 | 0.000 | |
| R-DME-2514856 | The phototransduction cascade | Vision | 12 | 6 | 0.961 | 1.687 | 0.000 | 0.025 | |||
| R-DME-5620920 | Cargo trafficking to the periciliary membrane | Vision | 15 | 4 | 0.956 | 1.683 | 0.000 | 0.018 | |||
| R-DME-5617833 | Cilium Assembly | Vision | 15 | 4 | 0.956 | 1.674 | 0.000 | 0.018 | |||
| R-DME-5620916 | VxPx cargo-targeting to cilium | Vision | 12 | 4 | 0.965 | 1.655 | 0.000 | 0.026 | |||
| R-DME-2514859 | Inactivation, recovery and regulation of the phototransduction cascade | Vision | 12 | 6 | 0.961 | 1.644 | 0.000 | 0.029 | |||
| R-DME-2187338 | Visual phototransduction | Vision | 14 | 6 | 0.957 | 1.644 | 0.000 | 0.025 | |||
| R-DME-76002 | Platelet activation, signaling and aggregation | Vision/Muscle activity | 47 | 9 | 0.784 | 1.640 | 0.000 | 0.024 | |||
| R-DME-71291 | Metabolism of amino acids and derivatives | Metabolism | 57 | 19 | 0.761 | 1.634 | 0.000 | 0.027 | |||
| R-DME-2672351 | Stimuli-sensing channels | Vision | 9 | 3 | 0.954 | 1.622 | 0.000 | 0.034 | |||
| R-DME-500792 | GPCR ligand binding | Vision | 14 | 4 | 0.920 | 1.618 | 0.002 | 0.034 | |||
| Network Analysis | PPI_BIOGRID | Head | PPI_BIOGRID M119 | Muscle activity | 33 | 16 | 0.872 | 1.711 | 0.000 | 0.004 | |
| PPI_BIOGRID M37 | Muscle activity | 71 | 20 | 0.767 | 1.683 | 0.000 | 0.004 | ||||
| PPI_BIOGRID M80 | Vision | 12 | 8 | 0.957 | 1.643 | 0.000 | 0.017 | ||||
*Non-Redundant