Fig 7. Schematic overview of specificity estimation using experimental data.

(A) BCR repertoires from 58 individuals with acute dengue infection are used to evaluate the performance of the clonal inference process. One of the individuals is chosen as the “base” (white plane). (B) Single sequences (gray-circles) are chosen randomly from each of the remaining individuals (gray planes) and added to the sequencing data from the base individual (white-circles). After clonal assignments, sequences from non-base individuals which are correctly determined to be singletons will be counted as true negatives (TN, check mark), while any grouping of these sequences into larger clones will be counted as false positives (FP, cross mark). Specificity is calculated as TN/(TN+FP).