Fig. 7.

EX-527 or 4-bromo-resveratrol (4bRSV) could not reverse the effect of resveratrol (RSV) on correcting cholesterol distribution. A: IB3 cells were treated with RSV (50 µM) in the absence and presence of EX-527 (1 µM) for 24 h and assessed for cholesterol distribution by filipin staining. Significance was determined by ANOVA and Newman-Keuls multiple-comparison test to determine significance between groups. Significance is shown for groups compared with vehicle (NT), *P < 0.05 (n = 5 experiments). Representative images are shown for each condition. B: IB3 cells were treated with RSV (50 µM) in the absence and presence of 4bRSV (15 µM) for 24 h and assessed for cholesterol distribution by filipin staining. Significance was determined by ANOVA and Newman-Keuls multiple-comparison test to determine significance between groups. Significance is shown for groups compared with vehicle (NT), *P < 0.05 (n = 5 experiments). Representative images are shown for each condition. Scale bars represent 50 µm.