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. 2020 Feb 7;9(3):1. doi: 10.1167/tvst.9.3.1

Figure 2.

Figure 2.

Comparison of immunostaining using isolated free-floating retinas and intact eyes. (A) Experimental procedure for immunostaining using isolated free-floating retinas; see Table 3 for details. (B) Experimental procedure for immunostaining using intact eyes; see Table 4 for details. After staining, the retina was isolated from the eye, and the flat-mounted retina was used to take images. (C, D) Tuj1 immunostaining using an isolated free-floating retina (upper) and an intact eye (lower) of C57BL/6 mice. Note that RGC axons were clearly stained with Tuj1 antibody in both cases. (E, F) Iba1 immunostaining using an isolated free-floating retina (upper) and an intact eye (lower) of ICR mice without CUBIC and melanin bleaching. Note that microglia were clearly stained with anti-Iba1 antibody in both cases. Scale bars, 200 µm (C, E) and 100 µm (D, F).