Table 3.

Detailed experimental procedure for the retina staining in Figure 2A

Day	Procedure
1	Isolation of the eye
	Fixation in 4% PFA at 4°C overnight
2	Washing with PBS, 10 min × 3
	Isolation and preparation of the free-floating retina
	Blocking with 0.5% Triton X-100 and 2% BSA for 2 h
	Incubation with first antibody at 4°C overnight
3	Washing with PBST, 10 min × 3
	Incubation with second antibody at RT for 4 h
	Washing with PBST, 10 min × 3
	Flat-mounting on slides
	Imaging using a microscope

BSA, bovine serum albumin; PBST, 0.5% Triton X-100/PBS; RT, room temperature.