Figure 3.

VEGFR1 was knocked down by lentiviral shRNA and downregulated VM-related proteins in melanoma cells. (A) Western blot of MUM-2B human melanoma cells collected at the logarithmic growth phase and subjected to western blot analysis. (n. 3). (B–D) Quantification of VEGFR1, CD144, and EphA2 proteins detected by immunoblot (A). Immunoblot densities were normalized to Gapdh as an internal control and compared with the density of the control. Bars depict the mean total intensity. (n. 3). ^*P < 0.05. (E) RT-PCR analysis of expression of the VEGFR1 gene in MUM-2B human melanoma cells. The expression of the gene for Gapdh was examined as a control. Bars depict the mean total mRNA. (n. 3). ^*P < 0.05.